AraShare科学

Product Information

Description:	Rabbit polyclonal antibody
Background:	ATPase Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF0 complex.
Synonyms:	ATPC2, F-ATPase gamma subunit 2
Immunogen:	KLH-conjugated synthetic peptide of ATPC2 derived from Arabidopsis thaliana AT1G15700.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Protein A purified
Reconstitution:	Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	43 / 36 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

Example 1

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

Example 2

1-9 is thylakoid membrane protein fromArabidopsis thalianaleaf.
1: Col-0 15ug of chlorophyll
2: Col-0 15ug of chlorophyll
3: Col-0 15ug of chlorophyll
4: Col-0 15ug of chlorophyll
5: atpc2 KO mutant
6: ATPC2 overexpression line 15ug
7: ATPC2 overexpression line 7.5ug
8: ATPC2 overexpression line 5ug
9: ATPC2 overexpression line 2.5ug .
Electrophoresis: 12.5% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:5000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (another company))
Detection: using chemiluminescence substrate and image were captured with CCD camera.