Product Information
Description: | Rabbit polyclonal antibody |
Background: | The D1 protein of Photosystem II (PSII), encoded by the PsbA genes, is an indispensable component of oxygenic photosynthesis. Due to strongly oxidative chemistry of PSII water splitting, the D1 protein is prone to constant photodamage requiring its replacement, whereas most of the other PSII subunits remain ordinarily undamaged. |
Synonyms: | PsbA, 32 kDa thylakoid membrane protein, Photosystem II protein D1 |
Immunogen: | KLH-conjugated synthetic peptide derived from N-terminal of D1 subunit in Arabidopsis thalianaATCG00020. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Protein A purified |
Reconstitution: | Reconstitution with 150µl of 0.01M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 39 / 26 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Vitis vinifera, Glycine max, Nicotiana tabacum, Populus trichocarpa, Brassica napus, Cucumis sativus, Spinacia oleracea, Brassica rapa, and 80-99% homologues with the sequence in Zea mays, Triticum aestivum, Oryza sativa Japonica Group, Hordeum vulgare, Gossypium raimondii, Panicum virgatum. |
Application Example
1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively. |