Product Information
Description: | Rabbit polyclonal antibody |
Background: | Type I chaperonins are large, double ring complexes invovled in mediating the folding of unfolded proteins. In chloroplasts, chaperonin complex is designated Cpn60 complex. In Arabidopsis, two Cpn60α subunits and four Cpn60β subunits are present.Cpn60β1 (AT1G55490) and Cpn60β2 (AT3G13470) is the main Cpn60β subunit in chloroplasts. This antibody recognizes both proteins. |
Synonyms: | CPN60B1/2 |
Immunogen: | KLH-conjugated synthetic peptide of CPN60B1/2 derived from Arabidopsis thalianaAT1G55490, AT3G13470. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150µl of 0.01 M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 64 / 58 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Brassica rapa, and 80-99% homologues with the sequence in Solanum tuberosum, Solanum lycopersicum, Gossypium raimondii, Populus trichocarpa, Medicago truncatula, Hordeum vulgare, Cucumis sativus. |
Application Example
Lane 1: 12 μg stromal protein from Arabidopsis thaliana leaf. |