Product Information
Description: | Rabbit polyclonal antibody |
Background: | In chloroplasts, transcription of plastid genes is mediated by two types of RNA polymerase: plastid-encoded RNA polymerase (PEP) and nuclear encoded RNA polymerase (NEP). PEP is composed of four core subunits (α, β,β’,β’’) and a promoter recognition subunit (σfactor). RpoC1 (ATCG00180) is the β’ subunit of PEP. |
Synonyms: | RpoC1 |
Immunogen: | KLH-conjugated synthetic peptide of RpoC1 derived from Arabidopsis thaliana ATCG00180. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150 µl of 0.01 M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 79 / 72 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Populus trichocarpa, Solanum tuberosum, Nicotiana tabacum, Solanum lycopersicum, Glycine max, Cucumis sativus, Gossypium raimondii, Vitis vinifera, Brassica rapa, Brassica napus, Spinacia oleracea, and 80-99% homologues with the sequence in Medicago truncatula. |
Application Example
20 μg total protein from Arabidopsis thaliana leaf. |