Product Information
Description: | Rabbit polyclonal antibody |
Background: | In chloroplasts, transcription of plastid genes is mediated by two types of RNA polymerase: plastid-encoded RNA polymerase (PEP) and nuclear encoded RNA polymerase (NEP). PEP is composed of four core subunits (α, β,β’,β’’) and a promoter recognition subunit (σfactor). RpoC2 (ATCG00170) is the β’’ subunit of PEP. |
Synonyms: | RpoC2 |
Immunogen: | KLH-conjugated synthetic peptide of RpoC2 derived from Arabidopsis thaliana ATCG00170. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150 µl of 0.01 M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 156 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus var. napus, Brassica rapa. |
Application Example
Lane 1: Thylakoid membrane protein from Arabidopsis thaliana leaf containing 5 μg of chlorophyll. |