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FBP / Anti-Fructose-1,6-bisphosphatase, chloroplastic antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY0403A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

During the photosynthesis, two isoforms of the fructose-1,6-bisphosphatase (FBPase), the chloroplastidial (cFBP1) and the cytosolic (cyFBP), catalyse the first irreversible step during the conversion of triose phosphates (TP) to starch or sucrose, respectively.

  Synonyms:

FBP, CFBP1, HCEF1, HIGH CYCLIC ELECTRON FLOW 1

  Immunogen:

KLH-conjugated synthetic peptide of FBP derived from Arabidopsis thaliana AT3G54050.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

45 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed , the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Spinacia oleracea, Brassica rapa, and 80-99% homologues with the sequence in Physcomitrium patens, Solanum tuberosum, Oryza sativa Japonica Group, Populus trichocarpa, Zea mays, Vitis vinifera, Cucumis sativus, Gossypium raimondii, Medicago truncatula, Nicotiana tabacum, Glycine max, Sorghum bicolor, Setaria viridis, Solanum lycopersicum.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

15 μg stromal protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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