Product Information
Description: | Rabbit polyclonal antibody |
Background: | Phosphoglycerate kinase is an ATP-generating enzyme that acts in the glycolytic, gluconeogenic, and photosynthetic pathways. Chl-PGK is encoded in the nucleus and translated to produce a 50 kDa precursor protein and is then processed into mature 43 kDa in the chloroplast. |
Synonyms: | PGK1, PHOSPHOGLYCERATE KINASE 1 |
Immunogen: | KLH-conjugated synthetic peptide of PGK1 derived from Arabidopsis thaliana AT3G12780. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 50 / 45 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Zea mays, Oryza sativa Indica Group, Solanum lycopersicum, Triticum aestivum subsp. Yunnanense, Triticum aestivum subsp. Tibeticum, and 80-99% homologues with the sequence in Solanum tuberosum. |
Application Example
Lane 1: 2.75 μg stromal protein from Arabidopsis thaliana leaf. |