敬请关注公众号
GAPA1 / Anti-Glyceraldehyde-3-phosphate dehydrogenase GAPA1, chloroplastic antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0408S
数量:999
-
+
产品或服务详情
产品或服务说明
配送或售后说明

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

GAPA1 is involved in the photosynthetic reductive pentose phosphate pathway. It catalyzes the reduction of 1,3-diphosphoglycerate by NADPH.

  Synonyms:

GAPA1, GAPA, GAPA-1, GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE A SUBUNIT, GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE A SUBUNIT 1

  Immunogen:

KLH-conjugated synthetic peptide of GAPA1 derived from Arabidopsis thaliana AT3G26650.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

GAPA1/2 (42 / 38) kDa, GAPB (48 / 43 ) kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Zea mays, Cucumis sativus, Populus trichocarpa, Solanum tuberosum, Glycine max, Physcomitrella patens.
The sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in GAPA2 (AT1G12900) and GAPB (AT1G42970), and 86% in GAPC1 (AT3G04120) and GAPC2 (AT1G13440). 
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 0.5 μg stromal protein from Arabidopsis thaliana leaf . 
Lane 2: 1 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 3: 2 μg stromal protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.