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RP-L1p / Anti-50S ribosomal protein L1p antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY0421A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

50S ribosomal protein L1p (RP- L1p family) binds directly to 23S ribosomal RNA.

  Synonyms:

RP-L1p, EMB3126, EMBRYO DEFECTIVE 3126, PLASTID RIBOSOMAL PROTEIN L1, PRPL1

  Immunogen:

KLH-conjugated synthetic peptide of RP-L1p derived from Arabidopsis thaliana AT3G63490.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

38 / 33 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Vitis vinifera, Cucumis sativus, Gossypium raimondii, Medicago truncatula, Populus trichocarpa, Spinacia oleracea, Glycine max, Solanum tuberosum, Nicotiana tabacum, Brassica rapa, Solanum lycopersicum, Brassica napus, and 80-99% homologues with the sequence in Physcomitrella patens.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

15 μg stromal protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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