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RPS1 / Anti-30S ribosomal protein S1 antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY0424A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Chloroplast ribosomal protein S1 (RPS1) is a heat-responsive protein and functions in protein biosynthesis in chloroplast.

  Synonyms:

RPS1, ARRPS1, PLASTID RIBOSOMAL PROTEIN S1, PRPS1, RIBOSOMAL PROTEIN S1

  Immunogen:

KLH-conjugated synthetic peptide of RPS1 derived from Arabidopsis thaliana AT5G30510.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

45 / 42 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Populus trichocarpa, Zea mays, Nicotiana tabacum, Brassica rapa, Solanum lycopersicum, Brassica napus, Cucumis sativus, Glycine max, Solanum tuberosum, Spinacia oleracea, Vitis vinifera, Setaria viridis, Oryza sativa Japonica Group, Sorghum bicolor, Hordeum vulgare, and 80-99% homologues with the sequence in Gossypium raimondii, Medicago truncatula.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

15 μg stromal protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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