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RP-L23.1/L23.2 / Anti-50S rpl23 ribosomal protein L23.1/L23.2 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0432S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Chloroplast ribosomal protein L23, a constituent of the large subunit of the ribosomal complex. RPL23 interacted with MDM2, forming a complex independent of the 80S ribosome and polysome.

  Synonyms:

RP-L23.1/L23.2

  Immunogen:

KLH-conjugated synthetic peptide of RP-L23.1 and L23.2 derived from Arabidopsis thalianaATCG00840, ATCG01300.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

11 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Spinacia oleracea, Gossypium raimondii, Cucumis sativus, Nicotiana tabacum, Oryza sativa Indica Group, Sorghum bicolor, Oryza sativa Japonica Group, Setaria viridis, Zea mays, Populus trichocarpa.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 11.4 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 2: 17.1 μg stromal protein from Arabidopsis thaliana leaf. 
Electrophoresis: Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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