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LQY1 / Anti-Low Quantum Yield of photosystem II 1 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0482S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

LQY1 is a Zn finger protein and shows disulfide isomerase activity in the chloroplast. LQY1 is mainly localized in stromal thylakoids and may be involved in maintaining PSII activity under high light by regulating repair and reassembly of PSII complexes. The absence of the LQY1 protein accelerates turnover and synthesis of PSII reaction center protein D1.

  Synonyms:

LQY1, LOW QUANTUM YIELD OF PHOTOSYSTEM II 1

  Immunogen:

KLH-conjugated synthetic peptide of LQY1 derived from Arabidopsis thaliana AT1G75690.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

16 / 12 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Zea mays.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-2 is thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 2.5 μg and 5 μg of chlorophyll, respectively. 
3 is thylakoid membrane protein from lqy1 mutant of Arabidopsis thaliana leaf containing 5 μg of chlorophyll. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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