Product Information
Description: | Rabbit polyclonal antibody |
Background: | Cytochrome f is a component of cytochrome b6/f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. |
Synonyms: | PetA, photosynthetic electron transfer A, Cyt f. |
Immunogen: | KLH-conjugated synthetic peptide of PetA derived from Arabidopsis thaliana ATCG00540. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150µl of 0.01M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 35 / 34 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Oryza sativa Japonica Group, and 80-99% homologues with the sequence in Nicotiana tabacum, Zea mays, Panicum virgatum, Sorghum bicolor, Glycine max, Vitis vinifera, Medicago truncatula f. tricycle, Gossypium raimondii, Medicago truncatula, Solanum tuberosum, Spinacia oleracea, Populus trichocarpa, Triticum aestivum, Hordeum vulgare subsp. Vulgare. |
Application Example
Example 1
1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively. |
Example 2
1-2 is thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 0.25 μg and 0.5 μg of chlorophyll, respectively. |