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SDH2 / Anti-Succinate dehydrogenase subunit SDH2, mitochondrial antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0561S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Mitochondrial succinate dehydrogenase complex is also called respiratory Complex II and it is an enzyme complex found in the inner mitochondrial membrane of eukaryotes. Succinate dehydrogenase complex catalyzes the oxidation of succinate to fumarate with the reduction of ubiquinone to ubiquinol. In Arabidopsis mitochondria, succinate dehydrogenase complex contains 8 subunits from SDH1-SDH8. SDH2 is encoded by AT3G27380/AT5G40650.

  Synonyms:

SDH2

  Immunogen:

KLH-conjugated synthetic peptide of SDH2 derived from Arabidopsis thaliana AT3G27380, AT5G40650.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

31 / 26 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

The sequence of the synthetic peptide used for immunization is 80% homologues with the sequence in SDH2-3 (AT5G65165).
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

10 μg mitochondria protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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