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Sar1 / Anti-Secretion-associated and Ras-related protein 1 antibody
价格:¥3100.00 ¥3880.00
积分:180 分
货号:PHY0505A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Two types of small GTP-binding proteins, ADP-ribosylation factor (Arf) and secretion-associated and Ras-related (Sar), are major regulators of vesicle biogenesis in intracellular traffic and are founding members of a growing family that also includes Arf-relatedproteins (Arp) and Arf-like (Arl) proteins. 
Sar1 functions as a molecular switch to control the assembly of protein coats (COPII) that direct vesicle budding from ER.

  Synonyms:

Sar1

  Immunogen:

KLH-conjugated synthetic peptide of Sar1 derived from Arabidopsis thaliana AT3G62560.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

22 / 22 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Gossypium raimondii, Nicotiana tabacum, Brassica rapa, Brassica napus, Medicago truncatula, Vitis vinifera, Zea mays, Solanum lycopersicum, Cucumis sativus, Oryza sativa Japonica Group, Triticum aestivum, and 80-99% homologues with the sequence in Solanum tuberosum, Physcomitrella patens.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 10 μg total protein from Arabidopsis thaliana leaf. 
Lane 2: 25 μg total protein from Arabidopsis thaliana leaf. 
Lane 3: 50 μg total protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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