返回首页 登录 注册 购物车
关于我们 通知公告 服务指南 联系我们
试剂耗材频道
搜产品 敬请关注公众号
总站导航 AraShare PlantShare ExpShare 植科头条 学术讲堂 求职招聘 仪器设备 试剂耗材 技术服务 试剂耗材 植物抗体 拟南芥抗体
NAD1 / Anti-NADH-ubiquinone oxidoreductase chain 1 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0512S
数量:999
- +
加入购物车 立即购买 收藏此商品

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Complex I is the largest protein complex of the oxidative phosphorylation system in mitochondrial and it catalyzes NADH-quinone oxidoreduction. Complex I represents the main entrance site for electrons into the respiratory electron transfer chain. In Arabidopsis, Complex I have at least 49 subunits and NAD1 (ATMG00516/ATMG01120/ATMG01275) is one of the subunit.

  Synonyms:

NAD1, NADH DEHYDROGENASE 1

  Immunogen:

KLH-conjugated synthetic peptide of NAD1A (ATMG01275), NAD1B (ATMG01120), NAD1C (ATMG00516) derived from Arabidopsis thaliana.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

36 / 30 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Vitis vinifera, Medicago truncatula, Oryza sativa Indica Group, Oryza sativa Japonica Group, Gossypium raimondii, Nicotiana tabacum, Triticum aestivum, Cucumis sativus, Brassica rapa subsp. oleifera, Glycine max, Zea mays subsp. mays, Zea mays, Solanum tuberosum, and 80-99% homologues with the sequence in Brassica napus, Physcomitrella patens.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

10 μg mitochondria protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


猜你喜欢 CSD2 / Anti-Ch...
¥ 2540.00 ¥ 3180.00
AtpB / Anti-Be...
¥ 3100.00 ¥ 3880.00
Arf1 /Anti-ADP...
¥ 2310.00 ¥ 2890.00
Actin / Anti-A...
¥ 3100.00 ¥ 3880.00
CSD2 / Anti-Ch...
¥ 2540.00 ¥ 3180.00
AtpB / Anti-Be...
¥ 3100.00 ¥ 3880.00
Arf1 /Anti-ADP...
¥ 2310.00 ¥ 2890.00
Actin / Anti-A...
¥ 3100.00 ¥ 3880.00