Product Information
Description: | Rabbit polyclonal antibody |
Background: | Complex I is the largest protein complex of the oxidative phosphorylation system in mitochondrial and it catalyzes NADH-quinone oxidoreduction. Complex I represents the main entrance site for electrons into the respiratory electron transfer chain. In Arabidopsis, Complex I have at least 49 subunits and NAD5 (ATMG00060/ATMG00513/ATMG00665) is one of the subunit. |
Synonyms: | NAD5, NADH DEHYDROGENASE SUBUNIT 5 |
Immunogen: | KLH-conjugated synthetic peptide of NAD5A (ATMG00513), NAD5B (ATMG00665), NAD5C (ATMG00060) derived from Arabidopsis thaliana. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 74 / 50 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Vitis vinifera, Physcomitrella patens, Oryza sativa Indica Group, Gossypium raimondii, Triticum aestivum, Sorghum bicolor, Oryza sativa Japonica Group, Zea mays subsp. mays, Nicotiana tabacum, Medicago truncatula, Brassica napus, Glycine max, Brassica rapa subsp. oleifera, Spinacia oleracea, and 80-99% homologues with the sequence in Cucumis sativus. |
Application Example
![]() | 7.5 μg mitochondria protein from Arabidopsis thaliana leaf. |