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PsbH / Anti-PsbH subunit of Photosystem II, N-terminal antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0610S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PsbH is an 8-kD phosphoprotein in the Photosystem II oxygen evolving core. The exact molecular function of PsbH has not been determined but it may play a role in mediating electron transfer between the secondary quinone acceptors, QA and QB.

  Synonyms:

PsbH, PHOTOSYSTEM II REACTION CENTER PROTEIN H

  Immunogen:

KLH-conjugated synthetic peptide derived from N-terminal of PsbH in Arabidopsis thalianaATCG00710.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

8 / 10 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Medicago truncatula, Brassica napus, Gossypium raimondii, Glycine max, Cucumis sativus, Zea mays, Oryza sativa Japonica Group, Triticum aestivum, Hordeum vulgare subsp. Vulgare, Nicotiana tabacum, Populus trichocarpa, Physcomitrella patens, Spinacia oleracea, Vitis vinifera, and 80-99% homologues with the sequence in Synechocystis sp. PCC 6803, Chlamydomonas reinhardtii.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-3 is thylakoid membrane protein from WT of Arabidopsis thalianaleaf containing 1 μg, 2 μg, and 5 μg of chlorophyll, respectively. 
4-6 is thylakoid membrane protein from PSII mutant of Arabidopsis thaliana leaf containing 1 μg, 2 μg, and 5 μg of chlorophyll, respectively. 
Electrophoresis: Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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