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PsbTc / Anti-PsbTc subunit of Photosystem II antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0614S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PsbTc subunit is encoded by the chloroplast genome and having a molecular mass of about 3.8 kDa. PsbTc can be identified as a low molecular mass component of the isolated CP47-RC subcomplex. Deletion of the psbT (c) gene results in increased sensitivity to photoinhibitory stress.

  Synonyms:

PsbTc, YCF8

  Immunogen:

KLH-conjugated synthetic peptide of PsbTc derived from Arabidopsis thaliana ATCG00690.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

3.8 / 4.6 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Zea mays, Brassica napus, Glycine max, Nicotiana tabacum, Solanum lycopersicum, Gossypium raimondii, Oryza sativa Japonica Group, Medicago truncatula, Triticum aestivum, Cucumis sativus, Vitis vinifera, and 80-99% homologues with the sequence in Chlamydomonas reinhardtii, Physcomitrella patens.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-2 is thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 2 μg, 4 μg of chlorophyll, respectively.
3 is thylakoid membrane protein from PSII mutant of Arabidopsis thaliana leaf containing 4 μg of chlorophyll. 
Electrophoresis: Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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