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CCaP1 / Anti-Vacuolar calcium-binding protein-related antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0641S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

AtCCaP1 acts as a mediator in response to continuous dark or gibberellic acid. In Arabidopsis thaliana a new type of Ca2+-binding protein, CCaP1, which consists of 152 amino acid residues, and binds (45)Ca2+ even in the presence of a high concentration of Mg2+.

  Synonyms:

CCaP1, AtCCaP1

  Immunogen:

KLH-conjugated synthetic peptide of CCaP1 derived from Arabidopsis thaliana AT1G62480.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

17 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 25 µg total protein from Arabidopsis thaliana leaf. 
Lane 2: 50 µg total protein from Arabidopsis thaliana leaf. 
Lane 3: 100 µg total protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.