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PIP2;2/3/4/6 / Anti-Plasma membrane aquaporin 2;2/3/4/6 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0673S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PIP2; 2/3/4/6 are members of the plasma membrane intrinsic protein subfamily PIP2. They localize to the plasma membrane and exhibit water transport activity in Xenopus oocyte.

  Synonyms:

PIP2; 2/3/4/6

  Immunogen:

KLH-conjugated synthetic peptide of PIP2; 2/3/4/6 derived from Arabidopsis thalianaAT2G37170, AT2G37180, AT5G60660, AT2G39010.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

30 / 27 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Zea mays, Glycine max, Cucumis sativus.
The sequence of the synthetic peptide used for immunization is 92% homologues with the sequence in PIP2;8 (AT2G16850) , PIP2;7 (AT4G35100), PIP2;5 (AT3G54820), PIP2;1 (AT3G53420) and 85% homologues with the sequence in PIP1;2 (AT2G45960) , PIP1;1 (AT3G61430), PIP1;3 (AT1G01620), PIP1;4 (AT4G00430), PIP1;5 (AT4G23400). 
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 20 μg soluble protein from Arabidopsis thaliana leaf. 
Lane 2: 50 μg soluble protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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