Product Information
Description: | Rabbit polyclonal antibody |
Background: | AP2σ is one of a subunit of the adaptor protein complex 2 (AP-2). The AP-2 complex has been generally thought to function as a heterotetramer, consisting of two large (a/A and b/B)( At4G11380), one medium (m/M)( At5g46630), and one small (s/S)( At1g47830) subunit(s). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. |
Synonyms: | AP2σ, Adaptor AP-2 17 kDa protein, Adaptor-related protein complex 2 subunit sigma, Clathrin assembly protein 2 small chain, Clathrin coat assembly protein AP17, Clathrin coat-associated protein AP17, Sigma2-adaptin. |
Immunogen: | KLH-conjugated synthetic peptide of AP2σ derived from Arabidopsis thaliana AT1G47830. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Applications: | Western Blot (1:1000-1:2000); IL |
Expected Results: | 17 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Zea mays. |
Application Example
Lane 1: 5 μg total protein from Arabidopsis thaliana leaf. |