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AP2σ / Anti-AP-2 complex subunit sigma antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0707S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

AP2σ is one of a subunit of the adaptor protein complex 2 (AP-2). The AP-2 complex has been generally thought to function as a heterotetramer, consisting of two large (a/A and b/B)( At4G11380), one medium (m/M)( At5g46630), and one small (s/S)( At1g47830) subunit(s). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome.

  Synonyms:

AP2σ, Adaptor AP-2 17 kDa protein, Adaptor-related protein complex 2 subunit sigma, Clathrin assembly protein 2 small chain, Clathrin coat assembly protein AP17, Clathrin coat-associated protein AP17, Sigma2-adaptin.

  Immunogen:

KLH-conjugated synthetic peptide of AP2σ derived from Arabidopsis thaliana AT1G47830.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000); IL 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

17 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Zea mays.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 5 μg total protein from Arabidopsis thaliana leaf. 
Lane 2: 10 μg total protein from Arabidopsis thaliana leaf. 
Lane 3: 50.32 μg total protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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