Product Information
Description: | Rabbit polyclonal antibody |
Background: | RELATED TO UBIQUITIN 1 (RUB1, AT1G31340) and RUB2 (AT2G35635) are redundant and both proteins are essential for early embryonic cell divisions. In addation, RUB3 (AT1G11980) may function as a stable post-translational protein modifier. |
Synonyms: | ARABIDOPSIS THALIANA RELATED TO UBIQUITIN 1, ATRUB1, NEDD8, RELATED TO UBIQUITIN 1, RUB1, RELATED TO UBIQUITIN 2, RUB2, UBIQUITIN 7, UBQ7. |
Immunogen: | KLH-conjugated synthetic peptide of RUB1, RUB2 derived from Arabidopsis thalianaAT1G31340, AT2G35635. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Applications: | Western Blot (1:1000-1:2000) |
Expected Results: | 17 kDa (RUB1), 17 kDa (UBQ7), 9kDa (RUB3) |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Zea mays, Brassica napus, Spinacia oleracea, Populus trichocarpa, Medicago truncatula, Sorghum bicolor, Vitis vinifera, and 80-99% homologues with the sequence in Physcomitrella patens. |
Application Example
Lane 1: 20 μg total protein from Arabidopsis thaliana leaf. |