Product Information
Description: | Rabbit polyclonal antibody |
Background: | P-type H+-ATPases are required for generation of proton gradient across the plasma membrane. AHA2 (AT4G30190) and AHA3 (AT5G57350) are localized to the plasma membrane. |
Synonyms: | AHA, ARABIDOPSIS THALIANA ARABIDOPSIS H(+)-ATPASE, ATAHA, H(+)-ATPASE, HA |
Immunogen: | KLH-conjugated synthetic peptide of AHA derived from Arabidopsis thaliana AT2G18960 (AHA1), AT4G30190 (AHA2), AT5G57350 (AHA3). |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Reference: | Gaston A. Pizzio, et al., Plant Physiology 2015, 167; 1541–1553. |
Application Information
Applications: | Western Blot (1:1000-1:2000) |
Expected Results: | 104 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | The sequence of the synthetic peptide used for immunization is 93% homologues with the sequence in AHA7 (AT3G60330), AHA8 (AT3G42640), AHA9 (AT1G80660), AHA6 (AT2G07560), AHA11 (AT5G62670), AHA4 (AT3G47950), and 86% homologues with the sequence in AHA10 (AT1G17260), AHA10 (AT2G24520), AHA5 (AT2G24520). |