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PAS2 / Anti-PASTICCINO2 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0853S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PASTICCINO2 (PAS2) is a member of the protein Tyr phosphatase-like family, is conserved among all eukaryotes and is characterized by a mutated catalytic site. PAS2 was localized in the cytoplasm of dividing cells but moved into the nucleus upon cell differentiation, suggesting that the balance between cell division and differentiation is regulated through the interaction between CDKA;1 (AT3G48750) and the antiphosphatase PAS2.

  Synonyms:

PAS2, PASTICCINO 2, PEP, PEPINO.

  Immunogen:

KLH-conjugated synthetic peptide of PAS2 derived from Arabidopsis thaliana AT5G10480.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

25 / 30 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa subsp. pekinensis.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 20 μg total protein from Arabidopsis thaliana leaf. 
Lane 2: 50 μg total protein from Arabidopsis thaliana leaf. 
Lane 3: 30 μg soluble protein from Arabidopsis thaliana leaf. 
Lane 4: 45 μg soluble protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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