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AUX1 / Anti-Auxin transporter protein 1, N-terminal antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0882S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

AUX1 is an auxin influx transporter. AUX1 resides at the apical plasma membrane of protophloem cells and at highly dynamic subpopulations of Golgi apparatus and endosomes in all cell types. AUX1 action in the lateral root cap and/or epidermal cells influences lateral root initiation and positioning. Shoot supplied ammonium targets AUX1 and inhibits lateral root emergence.

  Synonyms:

AUX1, ATAUX1, AUXIN RESISTANT 1, MAP1, MODIFIER OF ARF7/NPH4 PHENOTYPES 1, PIR1, WAV5, WAVY ROOTS 5.

  Immunogen:

KLH-conjugated synthetic peptide derived from N-terminal of AUX1 in Arabidopsis thalianaAT2G38120.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

54 / 48 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 10 μg total protein from Arabidopsis thaliana leaf. 
Lane 2: 20 μg total protein from Arabidopsis thaliana leaf. 
Lane 3: 40 μg total protein from Arabidopsis thaliana leaf. 
Electrophoresis: 12% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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