Background:

AtMAP65-1 plays a role in stabilizing anti-parallel microtubules in the central spindle at anaphase to early cytokinesis but is not essential at the midline of the phragmoplast at later stages. The timing with which the MAP65-1 was targeted to the spindle appears to be regulated by a phosphorylation sensitive switch. AtMAP65-1 enhances microtubule polymerization and promotes nucleation and stabilizes microtubules against cold treatment and dilution. The Arabidopsis contains nine MAP65 proteins: MAP65-1 (AT5G55230); MAP65-2 (AT4G30910); MAP65-3(AT5G51600); MAP65-4(AT3G55210); MAP65-5(AT2G35600); MAP65-6(AT2G00910); MAP65-7(AT1G15360); MAP65-8(AT1G27920); MAP65-9(AT5G61760).

  Synonyms:

MAP65-1, AtMAP65-1, MICROTUBULE-ASSOCIATED PROTEINS 65-1.

  Immunogen:

KLH-conjugated synthetic peptide of AtMAP65-1 derived from Arabidopsis thalianaAT5G55230.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Reference:

Mao et al., Plant Physiology 2005, 138; 654-662.

  Expected Results: 

66 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.