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KINβ2 / Anti-SNF1-related protein kinase regulatory subunit beta-2 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0172S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

The active SnRK1 (Sucrose non-fermenting-1-related protein kinase) form is usually a heterotrimeric complex. Subcellular localization and specific target of the SnRK1 kinase are regulated by specific beta subunits. In Arabidopsis, there are at least seven genes encoding beta subunits, AKINbeta2, one of the SnRK1 kinase is involved in the specificity of interaction of the kinase with the cytosolic nitrate reductase.

  Synonyms:

KINβ2, AKINB2

  Immunogen:

KLH-conjugated synthetic peptide of KINβ2 derived from Arabidopsis thalianaAT4G16360.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

29 / 34 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 20 μg total protein from Arabidopsis thaliana leaf. 
Lane 2: 40 μg total protein from Arabidopsis thaliana leaf. 
Lane 3: 3 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 4: 6 μg stromal protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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