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RPL13B / Anti-60S ribosomal protein L13-1 antibody
价格:¥3100.00 ¥3880.00
积分:180 分
货号:PHY0182A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

60S ribosomal protein L13-1 is localized in the cytoplasm and belongs to the ribosomal protein L13e family. In Arabidopsis, L13e family contains three proteins (AT3G49010, AT5G23900, AT3G48960).

  Synonyms:

RPL13B, 40S RIBOSOMAL PROTEIN, ATBBC1, BBC1, BREAST BASIC CONSERVED 1, RSU2

  Immunogen:

KLH-conjugated synthetic peptide of L13-1 derived from Arabidopsis thalianaAT3G49010.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

24 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Zea mays, Populus trichocarpa, Brassica napus, Brassica napus, Brassica rapa, Solanum tuberosum.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 25 µg total protein from Arabidopsis thaliana leaf. 
Lane 2: 50 µg total protein from Arabidopsis thaliana leaf. 
Lane 3: 100 µg total protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.