Product Information
Description: | Rabbit polyclonal antibody |
Background: | V-ATPases are multisubunit membrane protein complexes that use the energy provided by ATP hydrolysis to generate a proton gradient across various intracellular and plasma membranes. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. |
Synonyms: | VHA-H ,V-ATPase, H |
Immunogen: | KLH-conjugated synthetic peptide of VHA-H derived from Arabidopsis thalianaAT3G42050. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150µl of 0.01M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:500-1:1000) |
Expected/apparent MW: | 50 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa, Brassica napus, and 80-99% homologues with the sequence in Medicago truncatula, Gossypium raimondii, Nicotiana tabacum, Cucumis sativus, Populus trichocarpa. |
Application Example
20 µg total protein from Arabidopsis thaliana leaf. |