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GLN1-2 / Anti-Glutamine synthetase cytosolic isozyme 1-2 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY4304S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

GLN1-2

  Synonyms:

GLN1-2

  Immunogen:

KLH-conjugated synthetic peptide of GLN1-2 derived from Oryza sativa Os03g0223400.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

39 kDa

  Confirmed Reactivity: 

Oryza sativa

  Predicted Reactivity:  

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Zea mays, Vitis vinifera, Triticum aestivum, Hordeum vulgare, Medicago truncatula, Sorghum bicolor, Glycine max, Populus trichocarpa, Nicotiana tabacum, Gossypium raimondii, Solanum lycopersicum, Solanum tuberosum, Brassica napus, Cucumis sativus, Spinacia oleracea.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

30 μg total protein from Oryza sativa leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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