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PTAC5 / Anti-PTAC5, putative, expressed antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY4390S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PTAC5

  Synonyms:

PTAC5

  Immunogen:

KLH-conjugated synthetic peptide of PTAC5 derived from Oryza sativa Os04g0514400.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

40 / 42 kDa

  Confirmed Reactivity: 

Oryza sativa

  Predicted Reactivity:  

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Physcomitrella patens, Spinacia oleracea, Gossypium raimondii, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Hordeum vulgare subsp. vulgare, Vitis vinifera, Zea mays, Brassica rapa, Brassica napus, and 80-99% homologues with the sequence in Populus trichocarpa, Medicago truncatula, Nicotiana tabacum, Setaria viridis.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 5 μl total chloroplast protein from Oryza sativa leaf.
Lane 2: 10 μl total chloroplast protein from Oryza sativa leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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