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CPSAR1 / Anti-GTP-binding protein OBGC, chloroplastic antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY0927S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

ATOBGC is a chloroplast protein which is close to the Obg nucleotide binding protein subfamily and displays GTPase activity. ATOBGC is required for the normal organization of mature thylakoid stacks, and ultimately for embryo development. And it is involved in thylakoid membrane biogenesis and functions primarily in plastid ribosome biogenesis during chloroplast development.

  Synonyms:

CPSAR1, ATOBGC, ATOBGL, CHLOROPLASTIC SAR1, EMB269, EMB3138, EMBRYO DEFECTIVE 269, EMBRYO DEFECTIVE 3138, OBG A-2, OBG-LIKE PROTEIN.

  Immunogen:

KLH-conjugated synthetic peptide of CPSAR1 derived from Arabidopsis thaliana AT5G18570.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

76 / 72 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 3 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 2: 6 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 3: 12 μg stromal protein from Arabidopsis thaliana leaf. 
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.