Product Information
Description: | Rabbit polyclonal antibody |
Background: | The eukaryotic regulatory protein 14-3-3 is involved in many important plant cellular processes including regulation of nitrate assimilation through inhibition of phosphorylated nitrate reductase (pNR) in darkened leaves. 14-3-3ω is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes. |
Synonyms: | 14-3-3ω, 14-3-3 PROTEIN G-BOX FACTOR14 OMEGA, 14-3-3OMEGA, GENERAL REGULATORY FACTOR 2, GF14 OMEGA, GRF2. |
Immunogen: | KLH-conjugated synthetic peptide of 14-3-3ω derived from Arabidopsis thaliana AT1G78300. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Applications: | Western Blot (1:1000-1:2000), Co-Immunoprecipitate |
Expected Results: | 29 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus. |
Application Example
![]() | Lane 1: 20 μg total protein from Arabidopsis thaliana leaf. |