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TL29 / Anti-Thylakoid lumenal 29 kDa protein, chloroplastic antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY1015S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

TL29 is one of the more abundant proteins in the thylakoid lumen of plant chloroplasts. Based on its sequence homology to ascorbate peroxidases, TL29 was suggested to be involved in the plant defense system against reactive oxygen species and consequently renamed to APX4.

  Synonyms:

TL29, APX4, ASCORBATE PEROXIDASE 4, THYLAKOID LUMEN 29.

  Immunogen:

KLH-conjugated synthetic peptide of TL29 derived from Arabidopsis thaliana AT4G09010.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

29 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Zea mays, Sorghum bicolor. 
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 1.25 μg, 2.5 μg, and 4 μg of chlorophyll, respectively. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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