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ACBP4 / Anti-Acyl-CoA-binding domain-containing protein 4 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY1026S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Arabidopsis acyl-CoA-binding proteins ACBP4 (AT3G05420)and ACBP5 (AT5G27630) are subcellularly localized to the cytosol and ACBP4 depletion affects membrane lipid composition. ACBP4 is expressed in all plant organs. It is involved in fatty acid transport. It plays a role in the biosynthesis of membrane lipids including galactolipids and phospholipids.

  Synonyms:

ACBP4, ACYL-COA BINDING PROTEIN 4, ATACBP4.

  Immunogen:

KLH-conjugated synthetic peptide of ACBP4 derived from Arabidopsis thaliana AT3G05420.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

73 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 20 μg total protein from Arabidopsis thaliana leaf. 
Lane 2: 50 μg total protein from Arabidopsis thaliana leaf. 
Lane 3: 22 μg soluble protein from Arabidopsis thaliana leaf. 
Lane 4: 40 μg soluble protein from Arabidopsis thaliana leaf. 
Electrophoresis: 10% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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