CPSF73-I / Anti-Cleavage and polyadenylation specificity factor subunit 3-I antibody
Rabbit polyclonal antibody
The CPSF proteins is the cleavage and polyadenylation specificity factor and is essential for the mRNA 3’-end processing. The Arabidopsis contains five CPSF proteins: CPSF160 (AT5G51660), CPSF100 (AT5G23880), CPSF73-I (AT1G61010), CPSF73-II (AT2G01730), and CPSF30 (AT1G30460).
CPSF73-I, AtCPSF73-I, CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR 73-I.
KLH-conjugated synthetic peptide of AtCPSF73-I derived from Arabidopsis thalianaAT1G61010.
Serum Peptide affinity form antibody available upon request at firstname.lastname@example.org.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
For more species homologues information, please contact tech support at email@example.com.
4 μg nuclear protein from Arabidopsis thaliana . Electrophoresis: 10% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1h. Blocking: 5% skim milk at RT or 4℃ for 1h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.