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CPSF73-I / Anti-Cleavage and polyadenylation specificity factor subunit 3-I antibody
价格:¥2600.00 ¥2890.00
积分:140 分
货号:PHY1043S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

The CPSF proteins is the cleavage and polyadenylation specificity factor and is essential for the mRNA 3’-end processing. The Arabidopsis contains five CPSF proteins: CPSF160 (AT5G51660), CPSF100 (AT5G23880), CPSF73-I (AT1G61010), CPSF73-II (AT2G01730), and CPSF30 (AT1G30460).

  Synonyms:

CPSF73-I, AtCPSF73-I, CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR 73-I.

  Immunogen:

KLH-conjugated synthetic peptide of AtCPSF73-I derived from Arabidopsis thalianaAT1G61010.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

77 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

4 μg nuclear protein from Arabidopsis thaliana . 
Electrophoresis: 10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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