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NAD2 / Anti-NADH dehydrogenase 2 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY1078S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Complex I is the largest protein complex of the oxidative phosphorylation system in mitochondrial and it catalyzes NADH-quinone oxidoreduction. Complex I represents the main entrance site for electrons into the respiratory electron transfer chain. In Arabidopsis, Complex I have at least 49 subunits. NAD2 is one of the subunit and contains two protein NAD2A (ATMG00285) and NAD2B (ATMG01320)

  Synonyms:

NAD2, NADH DEHYDROGENASE 2

  Immunogen:

KLH-conjugated synthetic peptide of NAD2A (ATMG00285), NAD2B (ATMG01320) derived from Arabidopsis thaliana

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

55 / 52 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Gossypium raimondii, Brassica napus, Brassica rapa subsp. oleifera, and 80-99% homologues with the sequence in Hordeum vulgare subsp. spontaneum, Medicago truncatula, Nicotiana tabacum, Triticum aestivum, Vitis vinifera, Glycine max, Cucumis sativus, Zea mays subsp. parviglumis, Zea mays subsp. mays, Oryza sativa Indica Group, Solanum tuberosum.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

7.5 μg mitochondria protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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