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NAD6 / Anti-NADH dehydrogenase 6 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY1079S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Complex I is the largest protein complex of the oxidative phosphorylation system in mitochondrial and it catalyzes NADH-quinone oxidoreduction. Complex I represents the main entrance site for electrons into the respiratory electron transfer chain. In Arabidopsis, Complex I have at least 49 subunits and NAD6 (ATMG00270) is one of the subunit.

  Synonyms:

NAD6, NADH DEHYDROGENASE 6

  Immunogen:

KLH-conjugated synthetic peptide of NAD6 derived from Arabidopsis thaliana ATMG00270.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

24 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Medicago truncatula, Brassica napus, Glycine max, Oryza sativa Japonica Group, Sorghum bicolor, and 80-99% homologues with the sequence in Cucumis sativus, Nicotiana tabacum, Vitis vinifera, Gossypium raimondii, Oryza sativa Indica Group.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

7.5 μg mitochondria protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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