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ATP8 / Anti-ATP synthase subunit 8, mitochondrial antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY1130A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Mitochondrial F0F1-ATP synthase is also called Complex V and it synthesis ATP from ADP and Pi using the proton motive force created by respiratory electron transport. ATP8 (ATMG00480/AT2G07707) is a subunit of mitochondrial F0F1-ATP synthase in Arabidopsis.

  Synonyms:

ATP8, YMF19, ATP SYNTHASE 8, ORFB

  Immunogen:

KLH-conjugated synthetic peptide of ATP8 derived from Arabidopsis thaliana ATMG00480, AT2G07707.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

18 / 17 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Brassica rapa, Cucumis sativus, and 80-99% homologues with the sequence in Triticum aestivum, Oryza sativa Japonica Group, Oryza sativa Indica Group, Sorghum bicolor, Gossypium raimondii, Zea mays.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

7.5 μg mitochondria protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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