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PSA2 / Anti-Protein PHOTOSYSTEM I ASSEMBLY 2, chloroplastic antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY1228S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PSA2 is a DnaJ/Hsp40 cysteine-rich domain superfamily protein, which involved in female gametophyte development. PSA2 harbors a conserved domain found in DnaJ chaperones where it has been shown to form a zinc finger and to have protein-disulfide isomerase activity. It is required for embryo sac development, the accumulation of photosystem I (PSI) during plant development, and chloroplast development.

  Synonyms:

PSA2, EDA3, EMBRYO SAC DEVELOPMENT ARREST 3

  Immunogen:

KLH-conjugated synthetic peptide of PSA2 derived from Arabidopsis thaliana AT2G34860.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

20 / 12 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, and 80-99% homologues with the sequence in Zea mays.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

1-3 is thylakoid membrane protein of Arabidopsis thaliana leaf containing 1 μg, 2 μg, and 5 μg of chlorophyll, respectively. 
Electrophoresis: Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.