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PetG / Anti-Cytochrome b6-f complex subunit 5 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY1229S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PetG is cytochrome b6-f complex subunit V which is the component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. It is required for either the stability or assembly of the cytochrome b6-f complex.

  Synonyms:

PetG

  Immunogen:

KLH-conjugated synthetic peptide of PetG derived from Arabidopsis thaliana ATCG00600.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

4 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Physcomitrella patens, Medicago truncatula, Nicotiana tabacum, Cucumis sativus, Spinacia oleracea, Oryza sativa Indica Group, Chlamydomonas reinhardtii, Populus trichocarpa.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

1-2 is thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 2 μg and 4 μg of chlorophyll, respectively. 
3 is thylakoid membrane protein from Arabidopsis mutant with low accumulation of Cyt b6f complex containing 4 μg of chlorophyll. 
Electrophoresis: Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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