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TIC40 / Anti-Protein TIC40, chloroplastic antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY1249S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

TIC40 is a protein in the chloroplast inner membrane translocon complex. It is involved in protein precursor import into chloroplasts and reinsertion of proteins from the chloroplast stroma into the inner membrane. Three components of the chloroplast protein translocon, Tic110(AT1G06950), Hsp93 (ClpC), and Tic40(AT5G16620), have been shown to be important for protein translocation across the inner envelope membrane into the stroma.

  Synonyms:

TIC40, PDE120, PIGMENT DEFECTIVE EMBRYO 120, TRANSLOCON AT THE INNER ENVELOPE MEMBRANE OF CHLOROPLASTS 40

  Immunogen:

Recombinant protein of TIC40 derived from Arabidopsis thaliana AT5G16620.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

49 / 43 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

Lane 1: 20 μg total protein from Arabidopsis thaliana leaf.
Lane 2: 10 μl total chloroplast protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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