Product Information
Description: | Rabbit polyclonal antibody |
Background: | ADH1 is a Zn-binding enzyme that acts as a dimer and relies on a NAD(P) co-factor to interconvert ethanol and acetaldehyde. ADH catalyzes the reduction of acetaldehyde using NADH as reductant. The protein undergoes thiolation following treatment with the oxidant tert-butylhydroperoxide. |
Synonyms: | ADH1, ADH, ALCOHOL DEHYDROGENASE, ALCOHOL DEHYDROGENASE 1, ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE, ATADH, ATADH1 |
Immunogen: | KLH-conjugated synthetic peptide of ADH1 derived from Arabidopsis thaliana AT1G77120. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Immunogen affinity purified |
Reconstitution: | Reconstitution with 150µl of 0.01 M sterile PBS. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 41 / 43 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Sorghum bicolor, Oryza sativa Japonica Group, Hordeum vulgare, Setaria viridis, Spinacia oleracea, Populus trichocarpa. |
Application Example
30 μg total protein from Arabidopsis thaliana leaf. |