Product Information
Description: | Rabbit polyclonal antibody |
Background: | Complex I is the largest protein complex of the oxidative phosphorylation system in mitochondrial and it catalyzes NADH-quinone oxidoreduction. Complex I represents the main entrance site for electrons into the respiratory electron transfer chain. In Arabidopsis, Complex I have at least 49 subunits and 51 kD (AT5G08530) is one of the subunit. |
Synonyms: | AT5G08530, 51 KDA SUBUNIT OF COMPLEX I, CI51, NDUFV1 |
Immunogen: | KLH-conjugated synthetic peptide derived from N-terminal of AT5G08530 protein in Arabidopsis thaliana AT5G08530. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 53 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Oryza sativa Japonica Group, Spinacia oleracea, Medicago truncatula, Glycine max, and 80-99% homologues with the sequence in Zea mays, Sorghum bicolor, Solanum lycopersicum, Populus trichocarpa, Physcomitrella patens, Vitis vinifera. |
Application Example
Lane 1: 10 μg mitochondria protein from Arabidopsis thaliana leaf. |