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ATP2 / Anti-ATP synthase subunit 2, mitochondrial, C-terminal antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY1414S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. It is encoded by a multigene family of three members (At5g08670, At5g08680, At5g08690). F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a1, b2 and c (9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF1 is attached to CF0 by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.

  Synonyms:

ATP2, AtpB, ATP synthase F1 sector subunit beta, F-ATPase subunit beta

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal of ATP2 protein in Arabidopsis thaliana AT5G08670, AT5G08680, AT5G08690.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

60 / 55 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Vitis viniferaSolanum lycopersicumNicotiana tabacumMedicago truncatulaGlycine maxPopulus trichocarpaZea maysSorghum bicolorOryza sativa Japonica GroupBrassica napus.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

Lane 1: 10 μg mitochondria protein from Arabidopsis thaliana leaf.
Electrophoresis:10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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