Product Information
Description: | Rabbit polyclonal antibody |
Background: | ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. It is encoded by a multigene family of three members (At5g08670, At5g08680, At5g08690). F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a1, b2 and c (9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF1 is attached to CF0 by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains. |
Synonyms: | ATP2, AtpB, ATP synthase F1 sector subunit beta, F-ATPase subunit beta |
Immunogen: | KLH-conjugated synthetic peptide derived from C-terminal of ATP2 protein in Arabidopsis thaliana AT5G08670, AT5G08680, AT5G08690. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Recommended Dilution: | Western Blot (1:1000-1:2000) |
Expected/apparent MW: | 60 / 55 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Vitis vinifera, Solanum lycopersicum, Nicotiana tabacum, Medicago truncatula, Glycine max, Populus trichocarpa, Zea mays, Sorghum bicolor, Oryza sativa Japonica Group, Brassica napus. |
Application Example
Lane 1: 10 μg mitochondria protein from Arabidopsis thaliana leaf. |