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ATP2 / Anti-ATP synthase subunit 2, mitochondrial, C-terminal antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY1414S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. It is encoded by a multigene family of three members (At5g08670, At5g08680, At5g08690). F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a1, b2 and c (9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF1 is attached to CF0 by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.

  Synonyms:

ATP2, AtpB, ATP synthase F1 sector subunit beta, F-ATPase subunit beta

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal of ATP2 protein in Arabidopsis thaliana AT5G08670, AT5G08680, AT5G08690.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

60 / 55 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Vitis vinifera, Solanum lycopersicum, Nicotiana tabacum, Medicago truncatula, Glycine max, Populus trichocarpa, Zea mays, Sorghum bicolor, Oryza sativa Japonica Group, Brassica napus.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

Lane 1: 10 μg mitochondria protein from Arabidopsis thaliana leaf.
Electrophoresis:10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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