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IAA7 / Anti-Auxin-responsive protein IAA7 antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY1464A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

IAA7 is a transcription regulator acting as repressor of auxin-inducible gene expression. It plays role in the control of gravitropic growth and development in light-grown seedlings.

  Synonyms:

IAA7, AUXIN RESISTANT 2, AXR2, IAA7, INDOLE-3-ACETIC ACID 7

  Immunogen:

KLH-conjugated synthetic peptide of IAA7 protein derived from Arabidopsis thalianaAT3G23050.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

26 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

Among species analyzed , the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Zea mays, Sorghum bicolor, Oryza sativa Indica Group, Oryza sativa Japonica Group, Setaria viridis, Hordeum vulgare, Triticum aestivum, Brassica napus, Brassica rapa, and 80-99% homologues with the sequence in Glycine max, Gossypium raimondii, Populus trichocarpa, Cucumis sativus, Medicago truncatula, Nicotiana tabacum.
The sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in IAA14 (AT4G14550), 94% homologues with the sequence in IAA17 (AT1G04250), 88% homologues with the sequence in IAA8 (AT2G22670), IAA9 (AT5G65670) and IAA16 (AT3G04730), 82% homologues with the sequence in IAA4 (AT5G43700) and IAA3 (AT1G04240).
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

6 μg nuclear protein from Arabidopsis thaliana.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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