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HYL1 / Anti-HYPONASTIC LEAVES1 antibody
价格:¥2540.00 ¥3180.00
积分:150 分
货号:PHY1465A
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Hyponastic leave 1 (HYL1, AT1G09700) is a double-stranded RNA-binding protein with 419 amino acids. HYL1 contains two double-stranded RNA (dsRNA) binding motifs, a nuclear localization motif, and a C-terminal repeat structure suggestive of a protein-protein interaction domain. HYL1 forms a complex with DICER-LIKE1 (DCL1, AT1G01040) and SERRATE (SE, AT2G27100) to process primary miRNA (pri-miRNA) into mature miRNA. It has been reported that HYL1 regulates the phase transition, establishment of stamen, and the adaxial–abaxial identity of leaf in Arabidopsis by controlling the biogenesis of different miRNA families.

  Synonyms:

HYL1, ATDRB1, DRB1, DSRNA-BINDING PROTEIN 1, HYPONASTIC LEAVES 1

  Immunogen:

KLH-conjugated synthetic peptide derived from C-terminal section of HYL1 protein in Arabidopsis thaliana AT1G09700.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

46 / 66 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

Lane 1: 20 μg total protein from Arabidopsis thaliana leaf. 
Lane 2: 40 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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