Product Information
Description: | Rabbit polyclonal antibody |
Background: | VHA-A is a catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. |
Synonyms: | VHA-A, VACUOLAR ATP SYNTHASE SUBUNIT A |
Immunogen: | KLH-conjugated synthetic peptide derived from C-terminal section of VHA-A protein in Arabidopsis thaliana AT1G78900. |
Form: | Lyophilized |
Quantity: | 150 µg |
Purification: | Serum |
Reconstitution: | Reconstitution with 150µl of sterile water. |
Stability & | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping: | The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above. |
Application Information
Applications: | Western Blot (1:1000-1:2000) |
Expected Results: | 69 kDa |
Confirmed Reactivity: | Arabidopsis thaliana |
Predicted Reactivity: | Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Vitis vinifera, Glycine max,Medicago truncatula, Spinacia oleracea, Brassica napus, Populus trichocarpa, Nicotiana tabacum, Cucumis sativus, Physcomitrella patens, Sorghum bicolor, Zea mays, Oryza sativa Japonica Group. |
Application Example
Lane 1: 40 μg total protein from Arabidopsis thaliana leaf. |