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CPK11 / Anti-Calcium-dependent protein kinase 11 antibody
价格:¥2310.00 ¥2890.00
积分:140 分
货号:PHY1497S
数量:999
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Product Information

  Description:

Rabbit polyclonal antibody

  Background:

CPK11 is one of calcium-dependent protein kinases, which are the best-characterized calcium sensors in plants. CDPKs regulate many aspects of plant growth and development as well as plant adaptation to biotic andabiotic stresses. CPK11 may play a role in signal transduction pathways that involve calcium as a second messenger. And it is the regulator of the calcium-mediated abscisic acid (ABA) signaling pathway.

  Synonyms:

CPK11, ATCDPK2, ATCPK11, CALCIUM-DEPENDENT PROTEIN KINASE 2, CDPK2

  Immunogen:

KLH-conjugated synthetic peptide of CPK11 derived from Arabidopsis thaliana AT1G35670.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at arashare01@qq.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

56 / 55 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, and 80-99% homologues with the sequence in Zea mays, Spinacia oleracea, Populus trichocarpa, Triticum aestivum.
For more species homologues information, please contact tech support at arashare01@qq.com.

 

Application Example

25 μg total protein from Arabidopsis thaliana leaf. 
Electrophoresis: 12% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.


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